sirtuin sirt 1 antibody  (Proteintech)

Journal: Molecules

Article Title: Anticancer Effects of Ursi Fel Extract and Its Active Compound, Ursodeoxycholic Acid, in FRO Anaplastic Thyroid Cancer Cells

doi: 10.3390/molecules26175309

Figure Lengend Snippet: Effects of UF extract and UDCA on the expression of angiogenic proteins in human FRO anaplastic thyroid cancer cells. The cells were treated with UF or UDCA at indicated concentrations for 48 h. The expression of angiogenic proteins TGF-β ( a ), VEGF ( b ), N-cadherin (N-Cad) ( c ), and SIRT-1(d) in the cells was determined by western blotting. ( A ) β-Actin was used as an internal control. The expression of each protein was calculated relative to β-actin expression. Data are representative of three independent experiments. ( B ) * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. normal cells. N, untreated cells; UF25, 25 μg/mL UF extract treatment; UF50, 50 μg/mL UF extract treatment; UDCA50, 50 μM/mL UDCA treatment; UDCA100, 100 μM/mL UDCA treatment; D, 2 nM/mL docetaxel treatment.

Article Snippet: Anti-vascular endothelial growth factor (VEGF) Ab (sc-146) was procured from Novus Biologicals (E. Briarwood Avenue, CO, USA), and anti-sirtuin-1 (Sirt-1) Ab (bc-0921R) was obtained from Bioss Antibodies Inc. (Woburn, MA, USA).

Techniques: Expressing, Western Blot

Journal: Biomolecules

Article Title: CRISPR/dCas9 Transcriptional Activation of Endogenous Apolipoprotein AI and Paraoxonase 1 in Enterocytes Alleviates Endothelial Cell Dysfunction

doi: 10.3390/biom11121769

Figure Lengend Snippet: Antibodies used for Western blot analysis.

Article Snippet: Mouse mAb , Human SIRT-1 , NBP1-51641 , 1:1000 , Novus Biologicals.

Techniques: Western Blot